Protein purification : principles, high resolution methods, and applications /
Contributor(s): Janson, Jan-Christer.
Series: Wiley series in methods of biochemical analysis: v. 54.Publisher: Hoboken, N.J. : John Wiley & Sons, c2011Edition: 3rd ed.Description: xiv, 517 p. : ill. (some col.) ; 29 cm.Content type: text Media type: unmediated Carrier type: volumeISBN: 9780471746614 (cloth); 9780471746614:; 0471746614 (cloth).Subject(s): Proteins -- Purification | Chromatographic analysis | ElectrophoresisDDC classification: 572.6Item type | Current library | Call number | Copy number | Status | Date due | Barcode | Item holds |
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Library Use Only | ATU Sligo Yeats Library Reference Collection | 572.6 JAN (Browse shelf(Opens below)) | 1 | Not for loan | 0075050 |
Includes bibliographical references and index.
Introduction to protein purification / B. Ersson, L. Rydén and J.-C. Janson -- Introduction to chromatography / J.-C. Janson and J.Å. Jönsson -- Gel filtration: Size exclusion chromatography / L. Hagel -- Ion exchange chromatography / E. Karlsson and I. Hirsh -- High-resolution reversed-phase chromatography of proteins / S.W. Pettersson -- Hydrophobic interaction chromatography / K.-O. Eriksson and M. Belew -- Immobilized metal ion affinity chromatography / L. Kågedal -- Covalent chromatography / F. Batista-Viera, L. Rydén and J. Carlsson -- Affinity chromatography / F. Batista-Viera, J.-C. Janson and J. Carlsson -- Affinity ligands from chemical combinatorial libraries / E. Carredano and H. Baumann -- Affinity ligands from biological combinatorial libraries / P.-Å. Nygren -- Membrane separations / J.K. Walter ... [et al.] -- Refolding of inclusion body proteins from E. coli / Z. Su, D. Lu and Z. Liu -- Purification of PEGylated proteins / C.J. Fee and J.M. Van Alstine -- Electrophoresis in gels / R. Westermeier -- Conventional isoelectric focusing in gel slabs and capillaries and immobilized pH gradients / P.G. Righetti, E. Fasoli and S.C. Righetti -- Two-dimensional electrophoresis in proteomics / R. Westermeier and A. Görg -- Protein elution and blotting techniques / R. Westermeier -- Capillary electrophoretic separations / W. Thormann -- High throughput screening techniques in protein purification / K.M. Lacki and E. Brekkan.
This is a state-of-the-art sourcebook on modern high-resolution biochemical separation techniques for proteins. It contains all the basic theory and principles used in protein chromatography and electrophoresis.