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Protein purification : principles, high resolution methods, and applications /

Contributor(s): Janson, Jan-Christer.
Series: Wiley series in methods of biochemical analysis: v. 54.Publisher: Hoboken, N.J. : John Wiley & Sons, c2011Edition: 3rd ed.Description: xiv, 517 p. : ill. (some col.) ; 29 cm.Content type: text Media type: unmediated Carrier type: volumeISBN: 9780471746614 (cloth); 9780471746614:; 0471746614 (cloth).Subject(s): Proteins -- Purification | Chromatographic analysis | ElectrophoresisDDC classification: 572.6
Contents:
Introduction to protein purification / B. Ersson, L. Rydén and J.-C. Janson -- Introduction to chromatography / J.-C. Janson and J.Å. Jönsson -- Gel filtration: Size exclusion chromatography / L. Hagel -- Ion exchange chromatography / E. Karlsson and I. Hirsh -- High-resolution reversed-phase chromatography of proteins / S.W. Pettersson -- Hydrophobic interaction chromatography / K.-O. Eriksson and M. Belew -- Immobilized metal ion affinity chromatography / L. Kågedal -- Covalent chromatography / F. Batista-Viera, L. Rydén and J. Carlsson -- Affinity chromatography / F. Batista-Viera, J.-C. Janson and J. Carlsson -- Affinity ligands from chemical combinatorial libraries / E. Carredano and H. Baumann -- Affinity ligands from biological combinatorial libraries / P.-Å. Nygren -- Membrane separations / J.K. Walter ... [et al.] -- Refolding of inclusion body proteins from E. coli / Z. Su, D. Lu and Z. Liu -- Purification of PEGylated proteins / C.J. Fee and J.M. Van Alstine -- Electrophoresis in gels / R. Westermeier -- Conventional isoelectric focusing in gel slabs and capillaries and immobilized pH gradients / P.G. Righetti, E. Fasoli and S.C. Righetti -- Two-dimensional electrophoresis in proteomics / R. Westermeier and A. Görg -- Protein elution and blotting techniques / R. Westermeier -- Capillary electrophoretic separations / W. Thormann -- High throughput screening techniques in protein purification / K.M. Lacki and E. Brekkan.
Summary: This is a state-of-the-art sourcebook on modern high-resolution biochemical separation techniques for proteins. It contains all the basic theory and principles used in protein chromatography and electrophoresis.
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Holdings
Item type Current library Call number Copy number Status Date due Barcode Item holds
Library Use Only Library Use Only ATU Sligo Yeats Library Reference Collection 572.6 JAN (Browse shelf(Opens below)) 1 Not for loan 0075050
Total holds: 0

Includes bibliographical references and index.

Introduction to protein purification / B. Ersson, L. Rydén and J.-C. Janson -- Introduction to chromatography / J.-C. Janson and J.Å. Jönsson -- Gel filtration: Size exclusion chromatography / L. Hagel -- Ion exchange chromatography / E. Karlsson and I. Hirsh -- High-resolution reversed-phase chromatography of proteins / S.W. Pettersson -- Hydrophobic interaction chromatography / K.-O. Eriksson and M. Belew -- Immobilized metal ion affinity chromatography / L. Kågedal -- Covalent chromatography / F. Batista-Viera, L. Rydén and J. Carlsson -- Affinity chromatography / F. Batista-Viera, J.-C. Janson and J. Carlsson -- Affinity ligands from chemical combinatorial libraries / E. Carredano and H. Baumann -- Affinity ligands from biological combinatorial libraries / P.-Å. Nygren -- Membrane separations / J.K. Walter ... [et al.] -- Refolding of inclusion body proteins from E. coli / Z. Su, D. Lu and Z. Liu -- Purification of PEGylated proteins / C.J. Fee and J.M. Van Alstine -- Electrophoresis in gels / R. Westermeier -- Conventional isoelectric focusing in gel slabs and capillaries and immobilized pH gradients / P.G. Righetti, E. Fasoli and S.C. Righetti -- Two-dimensional electrophoresis in proteomics / R. Westermeier and A. Görg -- Protein elution and blotting techniques / R. Westermeier -- Capillary electrophoretic separations / W. Thormann -- High throughput screening techniques in protein purification / K.M. Lacki and E. Brekkan.

This is a state-of-the-art sourcebook on modern high-resolution biochemical separation techniques for proteins. It contains all the basic theory and principles used in protein chromatography and electrophoresis.

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